The West African Centre for Cell Biology of Infectious Pathogens (WACCBIP) has kicked off an intensive week-long hands-on training workshop on flow cytometry, an essential technique in modern biological research.
The workshop, which started on Monday, February 8, 2024, at WACCBIP, University of Ghana, attracted participants from diverse scientific backgrounds to equip them with comprehensive knowledge and practical skills in utilizing flow cytometry for diverse scientific endeavours.
Under the expert guidance of Dr Diana Prah, Dr Henrietta Mensah-Brown, and Armaah-Vedjesu Evans Nii, the workshop commenced with an engaging introduction to flow cytometry, elucidating its fundamental principles and applications. Throughout the day, participants delved into topics ranging from the basics of flow cytometry and acquisition techniques to understanding the components of a flow cytometer and the role of fluorescence in cytometry.
As the workshop unfolded, participants immersed themselves in hands-on sessions, exploring multicolour immunophenotyping, panel design, and the critical aspect of fluorescence compensation.
One of the highlights of the workshop was the practical analysis of infected parasite red blood cells (RBCs), demonstrating the real-world applications of flow cytometry in infectious disease research. Participants gained invaluable experience in sample preparation techniques and were introduced to the intricacies of data analysis using cutting-edge software such as FACSdiva and Flowjo.
On the third day of the workshop, the emphasis shifted towards advanced techniques, notably multiparameter labelling and fluorescence compensation. Attendees actively participated in laboratory exercises aimed at honing their skills in preparing compensation samples. Following this, an analysis was conducted on whole cells using an 8-color panel.
Reflecting on the ongoing workshop, Dr Prah expressed her enthusiasm, stating, "Flow cytometry is a strong, high-throughput method with applications in a variety of fields, including vaccines, cancer, and infectious diseases. However, successfully designing tests and using the machine necessitates a high level of technical and analytical skills. In this continuous flow cytometry training program at WACCBIP, students are guided through proper experimental design, machine usage, data acquisition, and data analysis related to flow cytometry. We expect that in the future, the students will be able to comfortably apply flow cytometry to their research."
With two more days of immersive learning ahead, participants eagerly anticipate further exploration into flow cytometry methodologies and applications. WACCBIP remains committed to fostering scientific excellence and innovation, paving the way for groundbreaking research in West Africa and beyond.
